向凤宁 教授
E-Mail：xfn0990@sdu.edu.cn
个人简介
向凤宁，博士生导师，教育部新世纪优秀人才，山东省杰出青年基金，山东省植物生理学会副理事长，中国植物生理学会理事，中国细胞生物学学会植物器官发生分委会委员，中国植物生理与分子生物学会女科学家分分委员。
教育背景
博士后 2006-2007 韩国首尔大学 植物分子生物学
博士 2000-2003 山东大学 发育生物学
硕士 1986-1989 山东大学 植物学
本科 1982-1986 青海师范大学 生物学
工作经历
2003-至今 山东大学 教授（博导）
1998-2003 山东大学 副教授
1997-1998 山东大学 讲师
科研方向（在细胞生物学及植物学专业招收博士后、博士生、硕博连读生、硕士生）
1、拟南芥离体干细胞中心重建的遗传与表观遗传调控
在离体条件下，植物体细胞可通过干细胞功能的获得和干细胞中心的重建不断地进行植株再生，因此，离体干细胞中心重建的分子机制是解析植物细胞全能性的理论基础。本方向利用离体培养体系模拟生长点微环境及干细胞中心的重建过程，对离体苗再生相关突变体进行遗传学、分子生物学、细胞生物学以及生物化学分析，研究重要功能基因及其表观遗传修饰在离体干细胞中心重建中的作用机制，解析控制离体干细胞中心重建的分子网络。
2、大豆高产及耐逆功能基因发掘及作用机制研究
大豆是我国战略性经济作物，我国已成为世界最大的大豆进口国，大豆产业安全面临危机。因此，发掘大豆高产（株型、育性）和耐逆（盐碱、干旱、高低温）关键基因，解析其作用机制，培育高产/耐逆大豆新品种刻不容缓。本实验室创建了稳定、高效的大豆基因转化平台、构建了大豆EMS突变体库、建立了完善的大豆图位克隆功能基因体系以及大豆基因功能研究平台。在此基础之上，利用大豆突变体及自然群体材料，结合遗传学、分子生物学、基因组学、细胞生物学等手段，定位及克隆大豆高产和耐逆基因，鉴定基因功能、解析其调控产量及耐逆性状的分子机制和遗传网络，为分子育种提供理论基础。
主持课题
1.大豆抗旱／耐盐重要功能基因发掘及种质创新，国家转基因重大专项，2018ZX0800914B，2018-2019
2.环境胁迫应答调控元件的分离鉴定，国家转基因生物新品种培育重大专项，2016ZX08010002-009，2016-2020
3.大豆产量及耐逆关键基因的分离与遗传调控机制研究，国家重点研发计划， 2016YFD0101902，2016-2020
4.微环境调控植物生长点干细胞中心维持与重建的分子网络，国家重大科学研究计划（“973”）项目，2013CB967300，2013-2017
5.植物干细胞维持和分化的分子机理研究，国家重大科学研究计划（“973”）项目，2007CB948203，2007-2011
6.大豆高产抗逆功能基因组研究，国家863项目，2013AA102602-4，2013-2017
7.金银花规范化种植基地优化升级及系列产品综合开发研究，国家科技支撑计划课题，2011BAI06B01，2011-2015
8.拟南芥miR159调控离体干细胞中心重建的分子机制研究，国家自然科学基金，31770317，2018-2021
9.大豆NAC膜结合转录因子GmNTLx在大豆耐盐中的作用机制研究，国家自然科学基金，31471515，2015-2018
10.拟南芥生长素响应因子ARF10 调控离体苗再生的分子机制，国家自然科学基金，31270328，2013-2016
11.拟南芥WRKY71转录因子对腋分生组织形成及侧芽发育的调控研究，国家自然科学基金，30970243，2010-2012
12.红豆杉体细胞杂种紫杉醇合成基因高效表达及其等位变异研究, 国家自然科学基金，30771116，2008-2010
13.高寒藏药材－－川西獐牙菜药效成分相关基因的转移和克隆，国家自然科学基金，30470154，2005-2007
14.大豆、棉花和苜蓿干细胞维持与分化关键基因发掘及应用, 山东省自然科学基金重大基础研究项目，ZR2018ZC0334，2018-2020
15.高羊茅抗逆相关膜结合转录因子基因克隆及其基因工程育种研究，山东省杰出青年基金项目，QJ200810，2009-2011
16.小麦体细胞杂交中的杂种优势及其在育种上的利用，教育部新世纪人才支持计划项目，NCET-05-0581，2006-2008
17.拟南芥WRKY71转录因子对腋分生组织形成及侧芽发育的调控研究，教育部博士点基金，913111006，2010-2012
18.大豆抗盐基因分离及耐盐碱转GmST1大豆新品系培育，山东省科技发展计划项目，2013GNC11010，2014-2016
19.转PcPCs/PcGCs基因富集重金属剪股颖新品种选育，山东省公关项目，2009GG10002001，2010-2012
20.拟南芥WRKY71转录因子对分枝发育的调控机制研究，山东省国际科技合作项目，2011176，2013-2015
研究成果
1.Shuo Li, Nan Wang, Dandan Ji, Wenxiao Zhang, Ying Wang, Yanchong Yu, Shizhen Zhao, Menghua Lyu, Juanjuan You, Yangyang Zhang, Luli Wang, Xiaofang Wang, Zhenhua Liu, Jianhua Tong, Langtao Xiao, Ming-yi Bai and Fengning Xiang*. A GmSIN1/GmNCED3s/GmRbohBs feed-forward loop acts as a signal amplifier that regulates root growth in Soybean exposed to salt stress. The Plant Cell. 2019. 31: 2107-2130
2.Shuo Li, Nan Wang,Dandan Ji, Zheyong Xue, Yanchong Yu, Yupei Jiang, Jinglin Liu, Zhenhua Liu and Fengning Xiang*. Evolutionary and functional analysis of membrane-bound NAC transcription factor genes in soybean. Plant physiology. 2016. 172: 1805-1820
3.Yanchong Yu1, Zhenhua Liu, Long Wang, Sang-Gyu Kim, Pil J. Seo,, Meng Qiao, Nan Wang, Shuo Li,Xiaofeng Cao, Chung-Mo Park and Fengning Xiang,*. WRKY71 accelerates flowering via the direct activation of FLOWERING LOCUS T and LEAFY in Arabidopsis thaliana. The Plant Journal. 2016. 85: 96–106
4.Zhenhua Liu†, Juan Li†, Qiang Li, Long Wang, Qing Lu, Yanchong Yu, Shuo Li, Ming-yi Bai, Yuxin Hu and Fengning Xiang*. Repression of callus initiation by the miRNA-directed interaction of auxin-cytokinin in Arabidopsis thaliana. The Plant Journal. 2016. 87: 391–40
5.Meng Qiao, Zhongjuan Zhao, Yuguang Song, Zhenhua Liu, Lingxue Cao, Yanchong Yu, Shuo Li and Fengning Xiang*. Proper regeneration from in vitro cultured Arabidopsis thaliana requires the miRNA directed action of an auxin response factor. The Plant Journal. 2012. 71, 14–22
6.Pil Joon Seo1, Fengning Xiang1(co-first author), Meng Qiao, Ju-Young Park, Sang-Gyu Kim, Yong-Hwan Lee, and Chung-Mo Park. The MYB96 Transcription Factor Mediates Abscisic Acid Signaling during Drought Stress Response in Arabidopsis. Plant Physiology. 2009. 151(1):275-89
7.Zhao Cuizhu, Qiao Meng, Yu Yanchong, Xia Guangmin, Xiang Fengning*. The effect of the heterologous expression of Phragmites australis γ-glutamylcysteine synthetase on the Cd2+ accumulation of Agrostis palustris. Plant Cell & Environment. 2010. 33: 877–887
8.Nan Wang, Wenxiao Zhang, Mengyin Qin, Shuo Li, Meng Qiao, Zhenhua Liu and Fengning Xiang,*. Drought tolerance conferred in soybean (Glycine max. L) by GmMYB84, a novel R2R3-MYB transcription factor. Plant & Cell Physiology. 2017. 58(10): 1764–1776
9.Tao Xue, Xuehuan Dai, Ruipu Wang, Junzhu Wang, Zhenhua Liu* and Fengning Xiang*. ARGONAUTE10 Inhibits In Vitro Shoot Regeneration Via Repression of miR165/166 in Arabidopsis thaliana. Plant & Cell Physiology. 2017. 58(10):1789-1800
10.Yanchong Yu, Long Wang, Jiacai Chen, Zhenhua Liu, Chung-Mo Park and Fengning Xiang,*. WRKY71 Acts Antagonistically against Salt-delayed Flowering in Arabidopsis thaliana. Plant & Cell Physiology. 2018, 59(2): 414–422
11.Yanling Liu, Zhongjuan Zhao, Zhongjuan Zhao, Zheyong Xue, Long Wang, Yunfei Cai, Peng Wang, Tiandi Wei, Jing Gong and Fengning Xiang,*. An Intron-less β-amyrin Synthase Gene is More Important on Oleanolic Acid Accumulation than its Paralog in Gentiana straminea. Scientific Report. 2016. 6:33364: 1-13
12.Long Wang, Zhenhua Liu, Meng Qiao, Fengning Xiang,*. miR393 inhibits in vitro shoot regeneration in Arabidopsis thaliana via repressing TIR1. Plant Science. 2018. 266:1-8
13.Tao Xue, Zhenhua Liu, Xuehuan Dai, Fengning Xiang*. Primary root growth in Arabidopsis thaliana is inhibited by the miR159 mediated repression of MYB33, MYB65 and MYB101. Plant Science. 2017. 262: 182–189
14.Xuehuan Dai, Zhenhua Liu, Meng Qiao, Juan Li, Shuo Li and Fengning Xiang,*. ARR12 promotes de novo shoot regeneration in Arabidopsis thaliana via activation of WUSCHEL expression. JIPB (Journal of Integrative Plant Biology). 2017. 59(10): 747–758
15.Dexin Wang, Yanchong Yu, Zhenhua Liu, Shuo Li, Zeli Wang,* and Fengning Xiang,*. Membrane-bound NAC transcription factors in maize and their Contribution to the oxidative stress response. Plant Science. 2016.250:30–39
16.Junfeng Wang, Cuizhu Zhao, Chang Liu, Guangmin Xia and Fengning Xiang*. Introgression of Swertia mussotii gene into Bupleurum scorzonerifolium via somatic hybridization. BMC plant biology. 2011. 11:71-81
17.Cuizhu Zhao, Jin Xu, Qiang Li, Shuo Li, Peng Wang and Fengning Xiang*. Cloning and characterization of a Phragmites australis phytochelatin synthase (PaPCS) and achieving Cd tolerance in tall fescue. PLoS ONE. 2014.9(8):1-12
18.Yuguang Song, Dandan Liu, Shuo Li and Fengning Xiang*. The dynamic changes of DNA methylation and histone modifications of salt responsive transcription factor genes in soybean. PLoS ONE. 2012.7(7):1-8
19.Fengning Xiang, Junfeng Wang, Chunhui Xu, Guangmin Xia*. The chromosome content and genotype of two wheat cell lines and of their somatic fusion product with oat. Planta. 2010. 231:1201–1210
20.Meng Qiao and Fengning Xiang*. A set of Arabidopsis thaliana miRNAs involve shoot regeneration in vitro. Plant Signaling & Behavior. 2013. e23479-1-3
21.Gaobin Pu, Bingqian Zhou & Fengning Xiang*. Isolation and functional characterization of a Lonicera japonica hydroxycinnamoyl transferase involved in chlorogenic acid synthesis. Biologia. 2017. 72(6): 608—618
22.Yanchong Yu, Nan Wang, Ruibo Hu and Fengning Xiang*. Genome-wide identification of soybean WRKY transcription factors in response to salt stress. Springer Plus. 2016. DOI 10.1186/s40064-016-2647-x
23.Yunfei Cai, Guanghua Liu, Yang Yu and Fengning Xiang*, Genotyping and metabolite characterization of somatic hybrids between Arabidopsis thaliana and Swertia mussotii. In vitro plant. 2015. 51:360–368
24. Zhenhua Liu, Wei Xin, Dandan Ji, Long Wang, Juan Li and Fengning Xiang*. GUS activity for miR165a/166b, REV and WUS/CLV3 in in vitro direct Arabidopsis thaliana shoot regeneration. Protoplasma. 2013. 250:1213–1218
25.Gaobin Pua, Peng Wang, Bingqian Zhou, Zhenhua Liu, Fengning Xiang*. Cloning and characterization of Lonicera japonica p-coumaroyl ester 3’-hydroxylase which is involved in the biosynthesis of chlorogenic acid. Biosci.Biotechnol.Biochem. 2013. 77（7）:1403-1409
26.Fangfang Zhang, Peng Wang, Guangwen Kang, Fengning Xiang*. Asymmetric protoplast fusions between Bupleurum scorzonerifolium Willd and Taxus chinensis var. mairei. Plant cell report. 2011. 30:1857–1864
27.Yanchong Yu, Zidong Li, Peng Wang, Fengning Xiang*. Genetic and biochemical characterization of somatic hybrids between Bupleurum scorzonerifolium and Gentianopsis paludosa. Protoplasma. 2012. 249:1029–1035
28.Li Jiang, Yunfei Cai, Guangmin Xia, Fengning Xiang*. Introgression of the heterologous nuclear DNAs and efficacious compositions from Swertia tetraptera into Bupleurum scorzonerifolium Willd. via somatic hybridization. Protoplasma. 2012. 249:737–745
29.Meng Qiao, Zhongjuan Zhao and Fengning Xiang*. Arabidopsis thaliana in vitro shoot regeneration is impaired by silencing of TIR1. Biologia Plantarum. 2012. 56 (3): 409-414
30.Wei Xin, Zhenhua Liu, Yuguang Song and Fengning Xiang*. Direct shoot regeneration from Arabidopsis thaliana shoot apical meristems. Biologia Plantarum. 2012. 56 (4): 601-606
31.Zhong-juan Zhao§, Yu-guang Song§, Yan-ling Liu§, Meng Qiao, Feng-ning Xiang*. The effect of elicitors on oleanolic acid accumulation and the expression of triterpenoid synthesis genes in Gentiana straminea. Biologia Plantarum. 2012.57 (1): 139-143
32.Junfeng Wang, Yanling Liu, Yunfei Cai, Guangmin Xia, Fengning Xiang*. Cloning and functional analysis of geraniol 10-hydroxylase, a cytochrome P450 from Swertia mussotii. Biosci.Biotechnol.Biochem. 2010. 74 (8):1583-1590
33.Yanling Liu, Yunfei Cai, Zhongjuan Zhao, Junfeng Wang, Jing Li, Wei Xin, Guangmin Xia, Fengning Xiang*. Cloning and functional analysis of aβ-amyrin Synthase Gene associated with oleanolic acid biosynthsis in Gentiana straminea. Biol. Pharm. Bull. 2009.32(5):818-824
34.Cai Yunfei, Yanling Liu, Zhenhua Liu, Feng Zhang, Xiang Fengning*, Xia Guangmin. High-frequency embryogenesis and regeneration of plants with high content of gentiopicroside from the Chinese medicinal plant Gentiana straminea Maxim. In vitro plant. 2009. 45:730–739
35.Lihua Zang, Yanling Liu, Jiaqing Liu, Qing Tian, Fengning Xiang*. An Improved Method for Testing Weak VOCs in Dry Plants with a Purge and Trap Concentrator Coupled to GC-MS. CHROMATOGRAPHIA. 2008. 68:351-356
专利
1. 专利名：大豆萌动胚真空渗透辅助的外源基因转化方法，完成人：向凤宁，姬丹丹，王鹏，2010，授权号：ZL201010223264.5
2. 专利名：一种农杆菌介导的甘薯不定芽转化方法，完成人：向凤宁，王鹏，赵翠珠，2009，授权号：ZL200910017332.X
3. 专利名：芦苇植物络合素基因PaPCS及其应用，向凤宁 赵翠珠 赵赫，2011，授权号：201110189821.0
4 专利名：大豆圣豆9号NAC转录因子基因GmST1及其应用，完成人：向凤宁，姬丹丹，李朔，国家发明专利，2013.10.16，授权号：ZL201210128273.5
5. 专利名：大豆圣豆9号NAC转录因子基因GmST2及其应用，完成人：向凤宁，姬丹丹，李朔，国家发明专利，2013.12.25，授权号：ZL201210128590.7
6. 专利名：一种对金银花不定芽转化获得转基因植株的方法，完成人：向凤宁，周冰谦，国家发明专利，2014.11.05.21，授权号：ZL201310284657.0
7. 专利名：大豆圣豆9号GmNAC4基因盐诱导启动子，完成人：向凤宁，杨丹，宋伟宪，李朔，刘振华，国家发明专利，2016.12.7，授权号：ZL201410621142X
9. 专利名：金银花HQT基因冷诱导启动子，完成人：向凤宁，由娟娟，李朔，刘振华，国家发明专利，2017.10.24，授权号：ZL201510613599.0
10. 专利名：大豆威廉姆斯82 中NAC膜结合转录因子基因GmNTL1及其应用，完成人：向凤宁，王楠，李朔，刘振华，国家发明专利，2018.7.12，授权号：ZL201610867847.9
11. 专利名：一种大豆圣豆9号GmNAC15基因的ABA诱导启动子，完成人：向凤宁，王楠，李朔，刘振华，国家发明专利，2018.7.11，授权号：ZL201611091573.5
17. 大豆威廉姆斯82 中NAC膜结合转录因子基因GmNTL7及其应用，完成人：向凤宁，王楠，李朔，刘振华，国家发明专利，2018.7.17，授权号：ZL201610867930.6
12. 专利名：一种大豆高效启动子盐诱导功能元件及其应用，完成人：向凤宁、王楠、李朔、刘振华，国家发明专利，2019.3.5，授权号：ZL201710112865.0
